Inexpensive. Non-sequential bases? No ligase required.
Long Fragment Reads. Start with high mol wt dna – 70-100k bases, sample prep that barcodes them, sequence it, and then informatically map it back to the fragment. Assembly then gives you 100kb base length. Chromosome phasing begins to become possible. [spiffy!]
Thus, you can do this over a genome, as well, it allows the maternal and paternal dna to be worked out.
Not planning to sell instruments: only going to be a sequence centre doing it as a service. 20,000 genomes by end of 2010. Big challenge is actually assembly. 60K cores in cluster, 30Pb diskspace.
Will partner with Genome centres. Yesterday signed an agreement to try a pilot with Broad. Will build genome centres around the world.
Trying to make sequencing ubiquitous. Send them sample, then click on link to get your sample.
Saves you capital on sequencing and then on compute infrastructure.
Will only do Humans! [I cracked up at this point.]
My comments: Ok, I missed the beginning, but the end was intersting. I totally don't understand the business model. By doing only human, they'll only find hospital customers.. which hospital will pay for them to build a data centre. I'll elaborate more on that in another post.
Labels: AGBT 2009